https://www.pierre-francois-roux.com/tags/bioinformatics/ Recent content in Bioinformatics on Pierre-François Roux Hugo -- gohugo.io en-us © 2021 Pierre-François Roux Wed, 27 Apr 2016 00:00:00 +0000 https://www.pierre-francois-roux.com/project/combined-dna-and-rna-sequencing-for-rna-editing-event-discovery/ Wed, 27 Apr 2016 00:00:00 +0000 https://www.pierre-francois-roux.com/project/combined-dna-and-rna-sequencing-for-rna-editing-event-discovery/ RNA editing refers to post-transcriptional processes that modify mature RNA sequences relative to their genomic DNA template. In animals, the best-characterized events are A-to-I substitutions mediated by ADAR enzymes and C-to-U substitutions mediated by APOBEC enzymes. These modifications can diversify transcript sequences, alter coding potential, affect RNA stability and contribute to gene regulation. The rise of high-throughput sequencing made transcriptome-wide RNA editing screens possible, but it also exposed a difficult methodological problem.